A simple, economic, accurate reverse phase isocratic RPHPLC method was developed for the quantitation of emtricitabine in capsule dosage form. The quantification was carried out using Gracesmart RP18, 5μ (100mm x 4.6mm). The mobile phase comprised of mixture of buffer, adjusted to pH 2.2 with dilute orthophosphoric acid and acetonitrile in the ratio of 88:12. The flow rate was 1.0ml/min with UV detection at 280nm. The method has been validated and proved to be robust. The calibration curve was linear in the concentration range of 50 to 500mcg/ml with coefficient of correlation 0.9981. The percentage recovery for emtricitabine was found to be 101.0%. The method is useful in the quality for the estimation of emtricitabine in capsule dosage form.