Ultrasonic velocity along with the density and viscosity are highly functional parameters to understand various interactions, like solute-solute, solute-solvent and solvent-solvent for the binary and/or ternary systems. This may define the reactivity and effectiveness of the system for particular applications. In the present paper, such measurements were carried on an Aminoglycoside antibiotic namely Gentamicin sulphate in aqueous solution for different concentrations varying from 0.001 to 0.1 mol.kg-1at temperatures 298.15, 303.15 and 308.15K. The acoustic impedance (Z), adiabatic compressibility (β), free length (Lf), free volume (Vf), internal pressure ( ), Relaxation time ( ), Absorption coefficient ( ), Cohesive energy (C.E), Gibb’s free energy ( ), Relative association ( ), Rao’s constant ( ), Wada’s constant ( ), Van deer Waal’s constant ( were also calculated. It was found that there is a certain degree of variation in these parameters with the change in concentration and temperature. These variations have been interpreted in terms of solute –solvent interactions.