Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

Abstract

Adrenomedullin: A Novel Vasoregulatory Peptide Hormone Enhances Invasion of Trophoblast on Uterine Endometrium-The First Trimester Human Trophoblast ACH-3P Cell Line on Early Pregnancy

Author(s): Kanchi Ravi Padma, Penchalaneni Josthna

Calcitonin Gene-related Peptide (CGRP), amylin and Adrenomedullin (ADM) belong to a distinctive group of calcitonin (CGRP-α)/CGRP-β) family peptides that have related biological outcome owing to their structure and cross-reactivity between receptors. We recently reported that the level of immune reactive ADM is significantly up regulated in pregnant rats during early implantation period. Furthermore, infusion of ADM antagonist in pregnant rats causes uteroimplantation growth restriction. The purpose of this study was to analyze the expression and possible role of ADM in ACH-3P first trimester cell lines. ADM is localized in syncytiotrophoblast, cytotrophoblast and endothelial cells throughout human pregnancy. This study also established that ADM enhances the invasion and migration of first-trimesterACH-3P cells. The present work mainly concerned with the cell line analysis to investigate cytotoxicity extent determination by 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay. The cell lines initially subjected to the MTT assay to determine the cytotoxicity effect of ADM22-52. DNA fragmentation is exposed by the presence of a multitude of DNA strand breaks in TUNEL assay by flow cytometric method. As a whole, the results from this study suggested that ADM have animperativerole in the physiology of human pregnancy via regulation of trophoblast invasion and migration.


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