Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

Abstract

Analytical Application of Different Spectrophotometric Methods for Simultaneous Determination of Norfloxacin and Tinidazole in their Pure Forms and their Pharmaceutical Preparation

Author(s): Eissa MS, Darweish E, Elghobashy MR, Shehata MA

Smart spectrophotometric methods were introduced for the simultaneous determination of Norfloxacin (NF) and Tinidazole (TZ) in pure forms and their tablet pharmaceutical formulation. The proposed methods under study are. Ratio Difference (RD) in which the amplitude difference at 273 and 306 nm for the determination of NF and TZ was measured. Mean Centering of Ratio spectra (MCR) in which the peak amplitude at 270 nm for the determination of NF and summation of the peak amplitudes at 270 and 304 nm for the estimation of TZ were measured. Dual Wavelength (DWL) in this method, wavelengths 210 and 287 nm were used for NF determination, 270 and 287 nm for TZ determination. Bivariate method (BIV) in which two wavelengths 280 and 305 nm for quantitative estimation of NF and TZ were used. All of the previous methods determined successfully NF and TZ in concentration ranges of 1-12 μg/ml and 3-30 μg/ml, respectively. Chemo metric assisted method Partial Least-Squares (PLS). All of the developed methods were validated using International Conference on Hormonization (ICH) guidelines and statistically compared to High Performance Liquid Chromatography (HPLC) reported method. The adopted methods can be applied for the regular analysis of NF and TZ mixture in QC laboratories.


PDF

FIND ARTICLES
SCImago Journal & Country Rank
 
Erkek öyle sert ve acımaz sikiyor ki bayan çaresiz bir şekilde tırtıl misali sikiş yataktan aşağı doğru kayıyor fakat hem adam hemde kadın aşırı mobil porno derecede zevk alıyorlar Uzun zamandır karısı ile ilgili planları olan türk porno adam onu başkaları sikerken hayal ediyor bu durumu karısından sikiş izle gizliyordu fakat bir gün adamın moralinin bozuk olduğunu anlayan karısı sikiş ona ne olduğunu sordu ve adamda ona her şeyi anlatmıştı karısı bu porno resim durumdan Büyük zenci yarağı sevenler için özel olarak eklenmiş videomuz türk porno kalın ve damarlı zenci siki arayıp bulamayanlar için özel olarak sikiş izle yayınlanmıştır filmde ise sarışın zengin sürtük büyük mobil sikiş kalın damarlı alet sevdiğini bir çok kez dile getirmiş