Dalbergia sissoo Roxb. popularly known as Sisam in India belongs to the family Fabaceae. It is an important plant of great economic importance. The objective of the present work was to compare different extract for the in-vitro anthelmintic potency of Dalbergia sissoo Roxb. leaves using Indian earthworms (Pheretima posthumad). The various extracts of Dalbergia sissoo roxb. includes petroleum ether, carbon tetrachloride, benzene and ethanol were used in different concentrations viz. 10, 25, 50 and 100 mg/ml of respectively for in-vitro testing of anthelmintic potency by determination of time of paralysis and time of death of worm. Piperazine citrate was used as standard. The result of present study of indicates Dalbergia sissoo potentiate to paralyze earthworm and also caused its death after some time. The shortest time of paralysis was observed at 100 mg/ml of carbon tetrachloride extract of Dalbergia sissoo which was 19min 14 sec and 48 min 15sec respectively. The results of the study were comparable to standard Piperazine citrate. The results of the present study demonstrate that carbon tetrachloride extract of Dalbergia sissoo leaves took less time to cause paralysis of the earthworm than other three extracts. Thus the present study demonstrates the traditional claim of Dalbergia sissoo Roxb. as an anthelmintic has been confirm as the ethanolic, benzene, petroleum ether and carbon tetra chloride extracts of leaves displayed activity against the earthworm used in study. While carbon tetrachloride extract demonstrated to highly potent anthelmintic activity compared to other three extract of Dalbergia sissoo Roxb.