Benzophenone and their derivatives are important and useful compounds with diverse pharmacological properties. In the present study, we have evaluated the in vitro antibacterial activity of new 2-azetidinonyl-5-(2-benzoylphenoxymethyl) -1,3,4-oxadiazole derivatives 6a-j using Gram positive and Gram negative bacterial strains. Minimal inhibitory concentrations (MIC) were determined in order to monitor the efficacy of the synthesized compounds. Certain compounds inhibit bacterial growth with low MIC (mg/mL) value. The most potent antibacterial compounds of this series were compounds 6a, 6d and 6g which have lower MIC value of 4.68 mg/ml in selected strains.