Stem infusion of Peltophorum pterocarpum Baker ex K. Heyne used in dysentery, for gargles, tooth powder and muscular pain. In present paper antimicrobial, antioxidant along with their phytochemical evaluation will be performed. Pet. ether, dichloromethane, ethyl acetate and methanol fractions were tested for their antimicrobial (using agar well diffusion method) and antioxidant activities (DPPH and FARP methods). Bioactive fraction was further subjected to GC-MS analysis for evaluation of its chemical composition. Maximum antimicrobial inhibition was demonstrated by ethyl acetate extract against Bacillus subtilis, Pseudomonas aeruginosa and Satphylococcus aureus (IZ 17.33 ± 0.33, 17.00 ± 0.00, with MIC of 31.25 and 125 μg/mL respectively). In antioxidant potentials, similar fraction showed significant activity i.e. 6 μg/mL with IC50 value of 96.70 ± 0.22 at 80 μg/mL concentrations, coinciding with FRAP results (1310.00 ± 0.00 mg AAE/g dw antioxidant potentials). Based on the results, ethyl acetate bioactive fraction was further subjected to GC-MS studies where 58 distinct peaks were observed. Valeranone (RT: 13.67 of Total: 28.00 %) identified as the major peak with β-sitosterol (area 5.51%), isosativene (RT: 10.70, area 2.77%), hexadecanoic acid (area 3.71%) and octadecanoic acid (area 2.39%).