Medical biotechnology is a new branch of biotechnology, which focuses on finding cures for diseases, and novel methods to inhibit pathogenic growth. The function of amniotic fluid is to protect the embryo from infection of foreign organisms and physical disturbances throughout its development within the egg. Minimum Inhibitory Concentration of this extra embryonic fluid is higher compared with standard antibiotics. In our research we studied the antimicrobial activity of amniotic fluid of the hen’s egg. Our aim was to check the antimicrobial activity of the same fluid in a laboratory setting, against a wide spectrum of bacteria, compare the zone of inhibition for the microbes with standard antibiotics, and to determine the storage capacity of the fluid in it the immobilized state. In our study the amniotic fluid extracted from hen’s egg are effective against Streptococcus, Staphylococcus, E. coli and Klebsiella and exhibiting MIC at 5% in 10 minutes. The extraction of amniotic fluid, its mode of action and immobilization by using natural dyes for increasing its effectiveness will be envisaged.