Coupled heterocycles are becoming interesting biological targets in bringing out potent pharmaceuticals for the treatment of various diseases. In view of this a series of 2-(1-benzofuran-2-yl)-quinoline-4-carboxylic acids and their resultant esters were evaluated for their antitubercular activity against H37Rv strain by Microplate Alamar Blue Assay (MABA) method. In silico cheminformatics studies on bioactivity prediction was carried out using mole information. The results revealed that the analogues 4a, 5b-c and 6a-b exhibited excellent antitubercular potential at concentration of 1.6 μg/ml. The compounds 4b-c and 7c exhibited good activity at 6.25 μg/ml and compounds 5a, 6c, and 7a-b showed significant activity at 12.5 μg/ml when compared with standard drugs pyrazinamide, streptomycin and ciprofloxacin. Benzofuran coupled quinoline moieties are vital for activity as they possess excellent drug-like characteristics, suggesting being potentially best inhibitor of H37Rv strain. The in silico bioactivity study also revealed that the compounds have significant interaction with the drug targets.