Several medicinal plants have been used in traditional health care systems to heal wounds. Wound infecting bacteria are one of the major factors that delays or prevent wound healing. In the present study, 3 medicinal plants those have been widely used in Siddha medicine to treat wounds namely, Erythrina variegata, Tamarindus indica and Datura metel were tested for their antibacterial activity against standard bacterial isolates which isolated from different sources including wounds, viz. Staphylococcus aureus sub species aureus (ATCC® 29213™), Pseudomonas aeruginosa (ATCC® 25668™), Escherichia coli (ATCC® 25922™) and Enterococcus faecalis (ATCC® 29212™). Leaf of E. variegata, seed coat of T. indica and fruit of D. metel were extracted with hexane, ethyl acetate and ethanol. Antibacterial activity was tested using two different methods, well diffusion and growth curve analysis in broth culture. Both methods revealed that all the extracts of T. indica and fruit of D. metel had inhibitory effect on S. aureus, a bacterial isolate from wound. Similarly extracts of E. variegata had antibacterial activity on E. faecalis and P. aeruginosa, urine and clinical isolates respectively. The results confirm the antibacterial property of the plant extracts and their possible role in wound healing. Further studies are being carried out to find out the active compounds that responsible for antibacterial activity.