Chlorophylls and carotenoids are two main pigments contained in microalgae. The pigments have important function as they serve as bioactive compounds with high nutritional value. In this study, chlorophyll and carotenoids contents of four microalgae strains isolated from local industry effluent were evaluated. The pigments were extracted in methanol and determined based on the absorbance of three wavelength: 470, 652.4 and 665.2 nm using Spectrophotometer UV-Vis. The results showed that three of the strains which were eukaryotic microalgae contained chlorophyll (a+b) with content of 4.037 ± 0.0875%, 4.967 ± 0.1050% and 3.880 ± 0.1866% of dry biomass for Micractinium ehime IPOME-1, Micractinium sp. CCAP IPOME-2 and Mychonastes rotundus IPOME-3 strains repectively. Under nitrogen starvation medium, chlorophyll (a+b) content of prokaryotic microalgae (Uncultured Oscillatoria sp IPOME-4 strain) decreased from 2.096 ± 0.0209% to 1.684 ± 0.0391% (decrease of 0.412%) and total carotenoid content of the strain increased with increase of 0.308%, from 0.773 ± 0.0289% to 1.081 ± 0.0390%.