Both Leea indica and Leea macrophylla are belonging to Leeaceae family. The aim of the present study was to detect different chemical groups of the methanol leaf extract of the plants (L. indica & L. macrophylla) and to compare the anti-diarrheal and antimicrobial activity of these extracts with four established market preparations. Phytochemical screening of extracts of L. indica, L. macrophylla confirmed the presence of alkaloids, glycosides, steroids, tannins, flavonoids, reducing sugars, and gums. In antidiarrheal activity test, the methanol extracts of the L. indica and L. macrophylla at the doses of 500 mg/kg and 250 mg/kg reduced the total number of stool in the mice to a considerable extent as well as increased the latency period in comparison to the control groups and the result was statistically significant but less in extent than market preparations. In the antibacterial activity test, it was observed that both the plant extracts did not produced significant zone of inhibition against all the pathogenic bacteria. On the other hand, in the anti-fungal activity test, it was observed that the extracts and market preparation showed significant antifungal activity against fungal species compared to fluconazole as a standard. In conclusion, methanol extracts of L. indica and L. macrophylla indicate that the plants contain effective bioactive compounds that can be used for the treatment of diarrheal and microbial diseases.