Anxiety disorders are the most common mental illness in the world and became a very important area of research interest in Psychopharmacology. Based on a traditional belief, Echium amoenum (EA) (Boraginaceous) dried flowers are used in Iran as an anxiolytic remedy and also as a mood enhancer. The present study was conducted with the aim of comparing the Anxiolytic-like effects of EA in the male and female rats. This study was carried out to compare the anxiolytic effects of the herbal tea of EA between male and female rats. In order to do this, 14 male rats weighing 150 to 200 grams were divided into two groups including control1, treatment1 (treated rats with herbal tea of EA) and 14 female rats weighing 100 to 150 grams were divided into two groups including control2, treatment2 (treated rats by herbal tea EA). Treatment groups rats (n = 7/group) had ad libitum access to the tea of Echium amoenum 0.3% (w/v), for a period of 24 hours before the test. Then, the behavior of rats was tested in order to sedative (locomotor activity) and anxiolytic (elevated plus maze) activity. All the data were given as Means±S. E. M. Data were analyzed by one-way ANOVA following by Tukey test. The study revealed that EA herbal tea in female rats has anxiolytic effects while in themale rats has sedative effects.