We have described a facile synthesis of novel 1,3-thiazolidine purine nucleosides. All these analogues are derived from the key intermediate N-tert-butoxycarbonyl-1,3-thiazolidine-2-ol, which was obtained from L-cysteine methylester hydrochloride. The tetrabutylammonium fluoride (TBAF) induced coupling of the 1,3-thiazolidine moiety with suitably protected purines afforded highly regioselective N-9 substituted purine nucleosides. All the newly synthesized products were characterized by 1H NMR, ES-MS and elemental analyses. Their antibacterial activity is reported.