A small tree, Tabernaemontana divaricata has been mentioned in Ayurvedic medicinal systems mainly for the treatment of diarrhea and cancer. Detection of amino acids was carried out from various extracts of T. divaricata. The study of amino acids was carried out using LC-MS technique. The amino acids from the extracts were compared with the standard amino acids. Total three amino acids were detected in ethanol and acetone extracts. Majority of amino acids were detected in water extract.