The aims of this research were analysis of the bioactive chemical products and evaluation of antibacterial and antifungal activity. Bioactives (chemical compounds often referred to as secondary metabolites) were analyzed using gas chromatography-mass spectroscopy (GC-MS) techniques, then the in vitro antibacterial and antifungal activity of the methanolic extract was evaluated. Proteus mirabilis bioactive compounds were identified in the methanolic extract of Proteus mirabilis. GC-MS analysis of Proteus mirabilis revealed the existence of the 1- Cyclopropyl-3,4-epoxyhex -5-en-1-yne, Benzene , (ethylsulfonyl), Pyrazolo[1.5-a]pyridine , 3-methyl-2-phenyl , 1- benzylindole , L-Proline , N-(cyclohexanecarbonyl)-,propyl , 3-Amino-7-nitro-1,2,4-benzotriazine 1-oxide , (+)- trans-3,4-Dimethyl-2 -phenyltetrahydro-1,4-thiazine , Figure: Isophthalic acid ,di(2-methoxyethyl) ester , Isophthalic acid ,di(2-methoxyethyl) ester , 4-Benzyloxy-N-methylamphetamine, 1-(2-Benzyloxy-4-methyl- cyclohex- 3-enyl)-1 -methyl-ethylamine , Ethyl 4-([(E)-(2-nitrophenyl) methylidene]amino) benzoate , Tolpropamine , Methcathinone , 4-Dehydroxy-N-(4,5-methylenedioxy -2-nitrobenzylidene)tyram. The results of anti-fungal activity produced by Staphylococcus aureus showed that the volatile compounds were highly effective to suppress the growth of Aspergillus terreus.