Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry and Computational Chemistry

Abstract

Determination of Vitamin C in some Pharmaceutical Dosage by UV-Visible Spectrophotometer Using Bromocresol Purple as a Chromogenic Reagent

Author(s): Isam Eldin H Elgailani, Rayan H Alghamdi

A developed and validated spectrophotometric method has been suggested for the analysis of vitamin C (ascorbic acid) of some drugs namely Aspirin-C, VC500 and Centrum in their drug formulation. The proposed method used the complexation of vitamin C with Bromocresol Purple (BCP) at pH 4.0. The optimum experimental conditions were studied carefully. The λmax of the complex formed (ascorbic acid and BCP) was occurred at 430 nm with absorptivity of 0.0398 L mol-1.cm-1. The linearity was obtained in the concentration range of 0.5-100 μg/ml for iron content in hemoglobin. The Limit of Detection (LOD) and Limit Quantification (LOQ) were found to be 0.677 and 2.051 μg/ml for the iron respectively and with R2 of 0.999. And the percentage recoveries ranged from 98.86-100.48%. Effects of analytical parameters on the analysis of vitamin C (Ascorbic acid) of the three drugs have been studied. This method can be applied for the estimation of vitamin C in vitamin C drugs in their pharmaceutical dosage.


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