Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry and Computational Chemistry

Abstract

Development and validation of a RP-HPLC Method for Simultaneous Estimation of Atenolol and Nitrendipine in Tablet Dosage Form

Author(s): M. V. Kumudhavalli, K. Anand Babu, B. Jayakar

A rapid high performance liquid chromatographic method has been developed and validated for the estimation of Atenolol and Nitrendipine simultaneously in combined dosage form. A Phenomenox C-18 column having dimensions of 4.6×250 mm and particle size of 5 μm in isocratic mode, with mobile phase containing a mixture of methanol: acetonitrile: water (40:40:20 v/v) (pH adjusted to pH 3.0 using orthophosphoric acid) was used. The mobile phase was pumped at a flow rate of 1.5 ml/min and the eluents were monitored at 235 nm. The selected chromatographic conditions were found to effectively separate Atenolol (R t: 2.61 min) and Nitrendipine (R t: 6.11 min) having a resolution of 11.097. The method was validated in terms of linearity, accuracy, precision, and specificity, limit of detection and limit of quantitation. Linearity for Atenolol and Nitrendipine were found in the range of 30-70 μg/ml and 6-14 μg/ml, respectively. The percentage recoveries for Atenolol and Nitrendipine ranged from 99.05- 100.51% and 99.14-101.60%, respectively. The limit of detection and the limit of quantitation for Atenolol were found to be 1.96 μg/ml and 5.95 μg/ml respectively and for Nitrendipine were found to be 0.34 μg/ml and 1.03 μg/ml, respectively. The method was found to be robust and can be successfully used to determine the drug content of marketed formulations.


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