Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry and Computational Chemistry

Abstract

Development and validation of UV-spectrophotometric methods for simultaneous estimation of amlodipine besylate and clopidogrel bisulfate in bulk and tablet dosage form

Author(s): Laxmileena D. Patil, Sachin V. Gudi, Deepali D. Jadav, Yogita A. Kadam, Sampada D. Dalvi and Pramod L. Ingale

Two simple spectrophotometric methods have been developed for simultaneous estimation of Amlodipine besylate (AB) and Clopidogrel bisulphate (CPS) from tablet dosage form. Method (I) is simultaneous equation method, wavelengths selected are 360 and 270 nm for AB and CPS respectively. Method (II) is the absorbance ratio method, based on the determination of graphical absorbance ratio at two selected wavelengths, one being the isoabsorptive point 329 nm and other being the absorption maxima of amlodipine besylate at 360 nm. Both drugs obeyed Beer’s law in the concentration range 5-45 μg/ml, correlation coefficient (r2 < 1). The accuracy and precision were determined and found to comply with ICH guidelines. Both the methods showed good reproducibility and recovery with % RSD in the desired range. The proposed methods can be successfully applied for the routine analysis of both the drugs from tablet dosage form.


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