To present study is to isolate, identify bacterial cultures from wound samples (Proteus, Escherichia coli, Staphylococcus aureus, Klebsiella and Bacillus) and to assess the antibacterial efficiency of aqueous and solvent extracts and silver nanoparticles of Pergularia daemia, Canna indica and Lawsonia inermis against bacterial strains. The pathogens were isolated, identified on the basis of Gram’s reaction and biochemical test. The aqueous and acetone extracts P. daemia, C. indica and L. inermis was used to test the antibacterial activity by agar well diffusion method. The silver nanoparticle was synthesized, characterized and confirmed using UV-Visible spectrum of each reaction mixtures at about 24 h of reaction using UV-Visible Spectrophotometer. The synergistic mixtures were prepared with different types of combination form of silver nanoparticles produced by P. daemia, L. inermis and C. indica. This study confirm the presence of E. coli, S. aureus, Bacillus species, Proteus species and Klebsiella species isolated from various wound infection samples. The L. Inermis showed maximum zone of inhibition at 25.2 ± 0.28 mm for E. coli and the minimum zone of inhibition of Proteus sp at 19.40 ± 0.56 mm. The antibacterial activity of water extract of C. indica. The maximum inhibition zone was observed against S. aureus. The silver nanoparticles were confirmed by color changes from dark green to brown color. The peak value was obtained at 454.5 nm for C. indica and 446 nm for L. inermis. The plants considered have found use in the field of nanotechnology.