Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry and Computational Chemistry

Abstract

Evaluation of Cytotoxicity and Apoptosis Inducing Effects of N-(5-mercapto-1,3,4-thiadiazol-2-yl)-2-phenylacetamide Derivatives as Caspase Enzymes Activators

Author(s): Ahmad Mohammadi-Farani, Leila Hosseinzadeh, Pouria Barazesh, Farahnaz Ahmadi, Alireza Aliabadi

A new series of previously reported cytotoxic 1,3,4-thiadiazole analogs as potential anticancer agents were evaluated for induction of apoptosis. Namely, activation of caspases 3, 8 and 9 were assessed in Human Prostate Cancer Cell Line (PC-3) as well as Human Colorectal Adenocarcinoma Cell Line (HT-29). Cytotoxicity of intended compounds was evaluated in PC-3, HT-29 and Human Breast Adenocarcinoma Cell Line (MCF-7) using 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl Tetrazolium Bromide (MTT) assay. Furthermore, activity of caspases 3, 8 and 9 were assessed in PC-3 and HT-29 cell lines and obtained results were compared to doxorubicin. Mitochondrial Membrane Potential (MMP) as well as capability of intracellular Reactive Oxygen Species (ROS) generation was also measured in these cell lines. A new series of recently reported cytotoxic 1,3,4-thiadiazole derivatives were studied mechanistically. Fortunately, the most of the investigated derivatives showed a significant increase in activity of caspases 3, 8 and 9, in PC-3 and HT-29 cell lines superior than doxorubicin. Only compound A (2-Cl) caused an increase in MMP in HT-29 cell line further than doxorubicin. None of the tested derivative did not tempted generation of reactive oxygen species in examined cell lines. Overall, the investigated derivatives could be introduced as potential anticancer lead compounds.


FIND ARTICLES
SCImago Journal & Country Rank
 
kız uzun zamandır masaj salonunda çalışıyor çok sulanan erkek oluyor ama porno izle bir türlü hiç birine vermiyor Ama sonunda genç paraya sıkışıyor ve bir porno resimleri sikiş için çok para teklif eden genç adama sonunda kabul ediyor ve evlerine giren amatör pornolar maskeli hırsız genç kadının odasındaki çekmecelerde para ararken kadının seksi iç sikiş çamaşırlarına denk geliyor Onları koklayarak yarağı taş gibi oluyor daha sonra genç porno fotoğrafları kadın odaya bir den bire girince hırsızı görüyor ve bağırmaya başlıyor Hırsız hızlıca porno hikayeleri kızın ağzını elleri ile kapatarak tam kızın kalçalarını kucağına alarak sevişiyor
ankara escort
mobile bitcoin casino
Casumo no deposit bonus bitcoin nedir?