In this study, we synthesized gold nanoparticles (AuNPs) from Auric-chloride (HAuCl4) by using tri sodium citrate as reducing agent. Their particle size range from 5-25 nm. Ultra violet spectrophotometry (UV-vis.) and Transmission Electron Microscope (TEM) used to characterize the synthesized AuNPs. Fabrics structure from REP and INTERLOCK made from cotton, PET and Lycra were used to be in medical purposes after treatments with AuNPs. The fabrics were treated with the prepared AuNPs with three concentrations 10, 20 and 25 mM to determine the best effective treated fabric structure from Atomic absorption and ultra violet protection factor (UBF) to be applied to toxicity test in vitro. Cytotoxicity of the prepared gold nanoparticles were evaluated using cell viability assay from MMT and IC50 values and these results confirm that we can use AuNPs safely in contact medical treatment with skin.