Antibacterial and antifungal agents are widely used in the management of infectious disease but most of them have developed resistance to micro-organism. To overcome this problem and to lower the side effects, many approaches can be utilized. Benzophenone derivatives are reported to have the broad spectrum of biological activities. In the present study we have screened benzophenone derivatives 5a-k against six bacterial species viz gram positive (Bacillus subtilis and Staphylococcus aureus) and gram negative (Pseudomonas aeruginosa, Salmonella typhi, Proteus vulgaris, Shigella flexneri) bacteria. Also compounds 5a-k were tested with six fungal species viz (Candida albicans, Aspergillus niger, Fusarium solani, Aspergillus flavus, Botrytis cinerea, Candida krusei). The obtained results showed that the synthesized compounds 5a-k exhibited moderate to good activities against all the tested strains. Compounds 5a, 5c, 5d, 5f and 5g have shown good antibacterial and antifungal activities against selected strains.