Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry and Computational Chemistry

Abstract

In vitro evaluation of MFS efflux pumps among multidrug resistant Acinetobacter baumannii isolated from patients hospitalized in ICU

Author(s): Azad khaledi, Farhad B. Hashemi and Abbas Bahador

Multiple drug resistance (MDR) in Acinetobacter baumannii strains is a rapidly rising phenomenon worldwide. Different mechanisms are responsible for this resistance and efflux pumps are among them. In A. baumannii resistance resulted from efflux pumps are mainly in Major facilitator super family (MFS) and Resistance nodulation cell division (RND) families. Therefore, this study has been conducted to study in vitro evaluation of MFS efflux pumps among multidrug resistant A. baumannii isolated from patients hospitalized in intensive care unit (ICU). In this cross-sectional study, 100 MDR isolates were selected from a total of 200 clinical A. baumannii isolated from ICU; Multiplex- RT- PCR methods was used to in vitro evaluation of MFS efflux pumps among multidrug resistant A. baumannii isolated from patients hospitalized in ICU. According to the susceptibility test, among 200 A. baumannii isolates, the prevalence rate of MDR, non MDR and extremely drug resistance (XDR) were 56%, 34% and 10% respectively. Abaye 0369 gene expression was observed in MDR and non-MDR isolates in presence of tetracycline which is an indication of its role in antibiotic resistance against tetracycline. In confirming the role of MFS transporters such as abaye 0369 in tetracycline resistance, functional studies should be designed. For assessing the effect of the expression of other studied MFS transporters in tetracycline resistance, their expression rate should be determined using Real Time PCR.


FIND ARTICLES
SCImago Journal & Country Rank
 
kız uzun zamandır masaj salonunda çalışıyor çok sulanan erkek oluyor ama porno izle bir türlü hiç birine vermiyor Ama sonunda genç paraya sıkışıyor ve bir porno resimleri sikiş için çok para teklif eden genç adama sonunda kabul ediyor ve evlerine giren amatör pornolar maskeli hırsız genç kadının odasındaki çekmecelerde para ararken kadının seksi iç sikiş çamaşırlarına denk geliyor Onları koklayarak yarağı taş gibi oluyor daha sonra genç porno fotoğrafları kadın odaya bir den bire girince hırsızı görüyor ve bağırmaya başlıyor Hırsız hızlıca porno hikayeleri kızın ağzını elleri ile kapatarak tam kızın kalçalarını kucağına alarak sevişiyor
ankara escort
mobile bitcoin casino
Casumo no deposit bonus bitcoin nedir?