Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry and Computational Chemistry

Abstract

In-Vitro Alpha-Amylase Inhibitor Activity and Antioxidant Activity of Actinobacteria Isolated from Marine Sediment Samples

Author(s): Elizabeth Mathew, Haimanti Mondal, Priyanka Kharat, Purbasha Saha, KV Bhaskara Rao

Marine environment is a diverse and untapped source of a wide variety of biologically active compounds. Among several organisms, marine actinomycete has been known to be a potential source for novel enzymes. Diabetes mellitus is a long term metabolic disorder characterized by hyperglycaemia. In the present study, marine actinobacteria isolated from the marine sediments of the coastal areas of Kothapattanam, Ongole, Andhra Pradesh, were used to screen for alpha-amylase inhibitor and antioxidant compounds, which are known to manage type 2 diabetes. A total of 20 actinobacteria were isolated from the marine sediments. Among the 20 isolates, an actinobacterial isolate designated VITMA1 showed 58.16% inhibition against alpha amylase enzyme. Further, VITMA1 also exhibited antioxidant activity. It showed 61.82% of DPPH radical scavenging activity, reducing power at 1.933 and a metal chelating activity of 20.33% respectively. The combined effect of antioxidant activity and alpha-amylase enzyme inhibition makes the secondary metabolite obtained from marine actinobacterial isolate, a potent anti-diabetic drug. Based on phenotypic characteristics, VITMA1 was identified upto the genus level as Streptomyces sp.


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