A bioactive phytochemical compound identified as 3-hydroxy-2,2-bis(6-methoxy-3-methyl-2,3-dihydrobenzofuran-2- yl) Propanal was isolated from the stem exudate of Brachystegia eurycoma Harms by making use of Column and Thin Layer Chromatography as separation techniques. It was then characterized by Infrared Spectroscopy, Proton Nuclear Magnetic Resonance Spectroscopy and Mass Spectrometry. The compound exhibited marked antibacterial activities against Pseudomonas aeruginosa, Streptococcus faecalis and Bacillus cereus. These results authenticate the use of the stem exudate of Brachystegia eurycoma Harms in the treatment of wounds and infections in herbal medicine in Nigeria. The results further suggest that a possible development of the compound in pharmaceutical industries would enable the compound to be used in the pharmaceutical treatment of infections and diseases caused by these organisms since it would be safer than synthetic ones.