Fungi were ubiquitous in nature. Fungi are eukaryotic, spore producing achlorophyllous, heterotrophic organisms. Saprophytic and facultative parasitic fungi present in the coastal and adjacent areas of the Puducherry. Different fungi were so far isolated from soil of different regions of Puducherry (India). Isolation of fungi from soil samples were carried out by serial dilution plate technique followed by their identification through microscopic and macroscopic method. Sabourard Dextrose Agar (SDA) media was used for the growth of fungi. In our findings, following fungi were isolated and identified which include: Aspergillus niger, Aspergillus flavus, Aspergillus terreus, Penicillium sp. Among all these isolated fungi, Aspergillus niger was more dominant and shows the fast and highest growth pattern at temperature 280C and pH 5.6.