Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry and Computational Chemistry

Abstract

Method development and validation for the estimation of valsartan in bulk and tablet dosage forms by RP-HPLC

Author(s): Srinath Nissankararao, Anil Kumar. A, Sravanthi. S. L. and Naga Silpa. J.

A simple, precise and accurate RP - High performance liquid chromatography (Reverse Phase - HPLC) method has been developed for the estimation of Valsartan in tablet formulations. The separation was achieved on a X terra ,RP-18(100mm Х 4.6mm 5μm) using a mobile phase consisting of a degassed mixture of water, Acetonitrile & Glacial acetic acid in the ratio of 550:450:1v/v with a flow rate of 2.0 mL/min. The mobile phase showed the most favorable chromatographic parameter for analysis. The detection of the constituent was done using UV detector at 248 nm. The retention time of valsartan was found to be 2.530 minutes. The method was validated for system suitability, precision, accuracy, linearity, robustness. The linearity range for Valsartan was found to be 4 – 12 μg / ml. The method is validated for accuracy, precision, linearity, specificity and robustness in accordance with ICH guidelines and revealed that the method established is specific, accurate, rapid, precise, reliable and reproducible for the method has been successfully used to analyze commercial dosage forms and its percentage recovery was found to be 99.65%.


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