The present investigation was designed to evaluate the protoscolicidal effects of Nectaroscordum tripedale L. essential oil against hydatid cyst protoscoleces. Different doses of N. tripedale essential oil (3.75 - 15 %) and were used for 10, 20, 30, and 60 minutes. The viability of hyatid cyst protoscolexes was assessed by Eosin test. The findings exhibited that N. tripedale essential oil showed considerable protoscolicidal effects on hydatid cysts protoscoleces; at the dose of 15% after 10 min of killed 100% of protoscoleces; while at the dose of 7.5% after 10 min of killed 100% of protoscoleces. This study revealed the potential of N. tripedale essential oil to produce a new scolicidal agent.