Fenugreek is a well known herb for its medicinal and cosmetic qualities such as hypocholesterlaenic, antibiotic and antifertility activities. The purpose of the work was to study the influence of environmental conditions on physico chemical properties of herb grown in four different geographic locations. The values of moisture, oil and total phenolic contents were recorded higher in fenugreek seed grown in Yemen as 5.47 ± 0.66, 7.04 ± 0.21 and 18.52 ± 1.80 respectively, where as ash content was higher in Egyptian seed with sodium, iron and copper content. Calcium and magnesium were the most abundant mineral in all the samples followed by potassium and sodium. The polunsturated linoleic and linoleic fatty acid were the major fatty acids. The ratio of C18:2/ C18:3 was lowest (0.957) in Egypt followed by Yemen, India and Saudia. The amino acid composition revealed the presence of most essentail amino acids in Yeman seeds. These wide variations in compositions indicated the effects of environmental, cultiavars regions and and biotic factors.