Capparis decidua (Gudhapatra) is traditionally used for curing a variety of ailments such as toothache, cough, asthma, intermittent fever and rheumatism in India. There are no reports of quantification of β- Sitosterol from this plant. Hence a TLC densitometric method has been developed and validated for quantification of this marker compound. β-Sitosterol was quantified from methanolic extract using the Solvent System of Toluene: Methanol (9: 1, v/v). The method was validated using ICH guidelines in terms of precision, repeatability and accuracy. Linearity range for β-Sitosterol was 80–480 ng/spot and its content was 0.0244 % w/w. This simple, precise and accurate method gave good resolution from other constituents of extract. It can be adopted for routine quality control of herbal material and formulations containing Capparis decidua.