Pseudomonas aeruginosa is an opportunistic pathogen that has an innate resistance to some antibiotics and disinfectants. These bacteria are one of the most common cause of nosocomial infections, commonly infects patients who experienced a decrease in the immune system and can cause various types of infections include surgical wound infections, burns, and infections of the urinary tract. The problem that almost occurred throughout the world this is bacterial resistance to many types of antibiotics. Some bacteria found become resistance to multi drug, which makes it difficult to select the antibiotics for the treatment of infections. This study aimed to determine the resistance pattern Pseudomonas aeruginosa isolated from clinical samples of patients treated at RSUP. Dr. M. Djamil Padang, West Sumatra Indonesia from August to October 95, 2015. Total value of bacteria test derived from sputum (35), swabs (22), Pus (23), urine (10), blood (3) and stool (2). The results of resistance tested by the agar diffusion method of Kirby-Bauer is ceftriaxone (43.16%), cefotaxime (42.10%), cefoperazone (36.84%), ofloxacin (31.58%), ciprofloxacin (28.42%), gentamycin (28.42%), ceftazidime (25,26%), Meropenem (24.21%), Ticarcilin (23.16%), Imipenem (23.16%), levofloxacin (20.0%), piperacillin (14.74%) and amikacin (8.42%). Out of 95 isolates, 30 isolates including multidrug resistant. The most effective antibiotics are amikacin (91.51%) and piperacillin (81.05%). The most resistant is ceftriaxone (43.16%) and cefotaxime (42.10%).