Abstract

Screening for active agent to anti-diarrhea by an evaluation of antimicrobial activities from three fractions of sappan wood (Caesalpinia sappan. L)

Author(s): Oktaf Rina, Sanusi Ibrahim, Abdi Dharma, Afrizal and Chandra Utami Wirawati

The object of this research was to evaluate and compare the antimicrobial activities of three fractions sappan wood (Caesalpinia sappan. L.) also known as sappan wood, that was methanol, ethyl acetate, and hexane fractions. The sappan wood was collected from traditional market, minced, extracted by methanol and fractionationtreatment by nhexane continues by ethyl acetate. These fractions were evaluated for antimicrobial activity against bacteria Escherichia coli and Staphylococcus aureus using the cup-plate agar diffusion method in Nutrient Agar medium to determine the minimum inhibitory concentration (MIC). The phytochemical of it extract was also analysis by test identification tests. The phytochemical of sappan wood proved the presence of phenolic, flavonoid, tannins and a little bit of terpenoid and saponin but no alkaloid and steroid compound. Out of the three fractions of sappan wood, the methanol fraction showed maximum average clear zone inhibition against. Antimicrobial activity from the three fraction(n-hexane, ethyl acetate, and methanol) of it wood showed that methanol fraction has the best value. The inhibition maximum concentration value of methanol extract of it to E.coli and S.aureus were 13,7± 0,6 mm and 16,3±2,1 mm, it recommended for use as agent antimicrobial to disease diarrhea earlier.

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