Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

Abstract

Screening of Drug Resistant Bacterial Pathogens from UTI Patients and Plasmid Curing Analysis in Ampicillin Resistant Klebsiella sp.

Author(s): Kaviyarasi V, Jamuna Devi J, Santhosh S, Dhandapani R

In the present study 19 urine samples were collected from Rio Lab, Global hospital located in Salem. The samples were serially diluted and 92 different colonies were isolated and identified. The isolates bacterial colonies were tested for its susceptibility to fourteen different antibiotics. Among the 14 antibiotics screened against the 92 isolates three isolates viz., Staphylococcus aureus (30%), Escherichia coli (70%) and Klebsiella sp. (50%) were resistant to erythromycin, amikacin and tetracycline. The concentration at which organism turns into sensitive was tested by increasing the concentration of the antibiotics. The spectrum of antibiotic activities showed that some antibiotics retain their efficiency on bacterial antibiotics such as amoxicillin, nitrofurantoin and ampicillin. The ampicillin resistant Klebsiella sp., was chosen for plasmid curing analysis. Plasmid curing analysis was performed using ethidium bromide at different concentrations viz., 75, 100 and 125 μg/ml to check whether the drug resistance was plasmid mediated. In the present study it was observed that the ampicillin resistant isolate was converted to sensitive after ethidium bromide treatment proving that resistance was plasmid mediated.


PDF

FIND ARTICLES
SCImago Journal & Country Rank
 
Erkek öyle sert ve acımaz sikiyor ki bayan çaresiz bir şekilde tırtıl misali sikiş yataktan aşağı doğru kayıyor fakat hem adam hemde kadın aşırı mobil porno derecede zevk alıyorlar Uzun zamandır karısı ile ilgili planları olan türk porno adam onu başkaları sikerken hayal ediyor bu durumu karısından sikiş izle gizliyordu fakat bir gün adamın moralinin bozuk olduğunu anlayan karısı sikiş ona ne olduğunu sordu ve adamda ona her şeyi anlatmıştı karısı bu porno resim durumdan Büyük zenci yarağı sevenler için özel olarak eklenmiş videomuz türk porno kalın ve damarlı zenci siki arayıp bulamayanlar için özel olarak sikiş izle yayınlanmıştır filmde ise sarışın zengin sürtük büyük mobil sikiş kalın damarlı alet sevdiğini bir çok kez dile getirmiş