Abstract

Simultaneous identification of caffeine�?¸ acetaminophen, sildenafilcitrate, tadalafil and vardenafil HCl in aphrodisiac traditional herbal medicines by Thin Layer Chromatography-Densitometry

Author(s): Reni Septiani and Sophi Damayanti

Thin Layer Chromatography (TLC)-Densitometry has been developed and validated for the identification of caffeine, acetaminophen, sildenafil citrate, tadalafil and vardenafil HCl in traditional medicine aphrodisiac simultaneously. Liquid-liquid extraction method was used for sample preparation and then continued by TLCDensitometry using TLC-Scanner-4 CAMAG. The method used silica gel 60 F254 as the stationary phase. Mobile Phase for TLC was ethyl acetate, n-propanol, NH3 25%(45:5:1). Rf values ofcaffeine, acetaminophen, sildenafil citrate, tadalafil and vardenafil HCl and tadalafil were 0.57, 0.69, 0.27, 0.77 and 0.42, respectively. Resolution value (R) of caffeine-acetaminophen, acetaminophen-tadalafil, sildenafil citrate-vardenafil HCl, and vardenafil HCl-caffeine was obtained in a value of 2.0, 1.5, 2.4 and 2.4, respectively. This method have limits of detection (LOD) of reference standards for caffeine, acetaminophen, sildenafil citrate, tadalafil and vardenafil HCl were 0.1475, 0.2273, 0.1346, 0.1951 and 0.2047 ïÂÂ�?­g/g spot whereas LOD of spiking were 0.3350, 0.3817, 0.1716, 0.3031 and 0.2659 ïÂÂ�?­g/g spot,respectively.

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