GET THE APP

Simultaneous RP-HPLC method for estimation of rupatadine fumarate and montelukast sodium in tablet dosage form | Abstract

Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

ISSN: 0975-413X
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission Systemof respective journal.

Abstract

Simultaneous RP-HPLC method for estimation of rupatadine fumarate and montelukast sodium in tablet dosage form

Author(s): Kumaraswamy.Gandla. Spandana.R, JMR Kumar, M.Lakshmi surekha, JVLN Sheshgiri Rao

A simple, selective, linear, precise, and accurate RP-HPLC method was developed and validated for the simultaneous estimation of Rupatadine and Montelukast from bulk and formulations. Chromatographic separation was achieved isocratically on a Hiber@ Lichrosphere ® C18 column (250×4.6 mm, 5 μ particle size) using a mobile phase, Methanol and Potassium Di Hydrogen Phosphate buffer and Acetonitrile (adjusted to pH 3.0 with 1% orthophosphoric acid) in the ratio of 50:30:20v/v/v. The flow rate was 1 ml/min and effluent was detected at 226 nm and 20μl of sample was injected. The retention time of Rupatadine and Montelukast were 2.48 min and 6.38 min. respectively. Linearity was observed in the concentration range of 5-30 μg/ml for both Rupatadine and Montelukast. Percent recoveries obtained for both the drugs were 99.53-100.16% and 97.63-98.95%, respectively. The percentage RSD for precision and accuracy of the method was found to be less than 2%. The method was validated according to the ICH guidelines with respect to specificity, linearity, accuracy, precision and robustness. The method developed can be used for the routine analysis of Rupatadine and Montelukast from their combined dosage form.


PDF

Select your language of interest to view the total content in your interested language

30+ Million Readerbase
SCImago Journal & Country Rank
Google Scholar citation report
Citations : 25868

Der Pharma Chemica received 25868 citations as per Google Scholar report

Der Pharma Chemica peer review process verified at publons
Der Pharma Chemica- Journals on pharmaceutical chemistry