Two simple, precise, accurate and economical UV spectrophotometric methods have been developed and validated for routine estimation of Guaifenesin and Salbutamol in tablet dosage form. Spectroscopic studies were carried out using double beam U.V.Spectrophotometer model JASCO. The marketed combination of Guaifenesin and Salbutamol that is salbusum forte from Local market. Method A employs formation and solving of simultaneous equation using 274 nm and 279 nm as two analytical wavelengths for both drugs in Distilled water, Whereas Method B involved formation of Q-absorbance equation at isobestic point ( 253 nm). Guaifenesin and Salbutamol shows Linearity in a concentration range of 10-50μg /ml. Recovery studies for guaifenesin 97.99 % and 99.22 % for salbutamol in case of simultaneous equation method confirming the accuracy of the proposed method.