A novel and simple approach to the synthesis of Schiff base ligand resulted from the condensation of Pramipexole (N6-propyl-4,5,6,7-Tetrahydro-,1,3-benzothiazole-2,6-diamine)with O-Vanillin (2-hydroxy-3-methoxy benzaldehyde ). Using this bidentate ligand, complexes of Cu(II), Ru(II) with general formula ML2 have been synthesized and its complexes were synthesized and characterized using different physic chemical studies as elemental analysis, FTIR, 1H NMR, conductivity, magnetic properties, thermal analysis, and their biological activities. The elemental analysis data suggest that stoichiometry to be 1:2 [M:L]. All the complexes are non electrolytic in nature as suggested by molar conductance measurements. Infrared spectral data indicate the co-ordination between ligand and central metal ion through deprotanated phenolic oxygen and azomethine nitrogen. In addition the authors have been screened the compounds for biological activity. It was found that the compounds have shown activity against the organisms like Escherichia coli, Klebsiella and Bacillus subtilis.