In the present study 4-substituted thiazolidinones derivatives were synthesized from 2- amino pyridine which was used as a starting material. In this first the pyridyl thiourea was synthesized using ammonium isothiocyanate and conc HCl. And from this (pyridyl thiourea) schiff’s bases were formed using different aldehydes and the Schiff’s bases obtained were cyclised into 4- substituted thiazolidinones using thioglycolic acid. The compounds obtained were purified by column chromatography using silica jel .The chemical structure of the compounds were confirmed using IR. 1HNMR and synthesized compounds are investigated for their antibacterial and antifungal activity against E.coli and S.aureus and C.albican using cup plate method. Compounds (4a, 4b) and 4d showed good activity.