In recent years area of sugar urea derivatives has established considerable attention because of the unique structural properties and activities that these compounds display. The urea-linkage at the anomeric center is a robust alternative to the naturally occurring O- and N-glycosidic linkages of oligosaccharides and glycoconjugates, and the natural products that have been identified to contain these structures show remarkable biological activity.With these approach a series of new glycosyl-3-o-tolyl carbamides were synthesized by the interaction of various glycosylamines with o-tolylisothiocyanate. The compounds obtained were identified and characterized by their physical, spectral and elemental analysis data. The synthesized compounds were screened for their in vitro antimicrobial activity against bacteria (S. aureus, E. coli, P. aeruginosa) and fungi (A. niger, T. viride). Some of these compounds exhibited moderate to good activity, whereas some were inactive.