Chalcones have been reported to present various biological activities such as anti-inflammatory, antioxidant, antitubercular, antibacterial activities. It is a basic moiety of many heterocyclic systems containing oxygen, sulphur and nitrogen. Nitrogen containing heterocyclic derivatives synthesized from chalcones have exhibited antiinflammatory, antioxidant, antitubercular, antibacterial activities. An attempt has been made to synthesize chalcones by the reaction of 4-acetylpyridine with various aromatic and heteroaromatic aldehydes. Further, chalcones derivatives were cyclised to pyrimidine analogs by using thiourea, urea and guanidine hydrochloride. The newly synthesized pyrimidine derivatives have been characterized by UV, IR, 1HNMR, 13CNMR, Mass spectra and elemental analysis and evaluated for their anti-inflammatory, antioxidant, antitubercular and antibacterial activities. It was found that 2-amino pyrimidine analog bearing 4-fluoro substitution on phenyl ring (3d) has exhibited excellent antitubercular activity at lowest concentration in the series moreover it has also exhibited good anti-inflammatory and antioxidant activities.