Schiff base complexes derived from DFMPM and glycine with Co(II), Ni(II) and Cu(II) were synthesized and characterized by IR, UV-Vis, 1HNMR, elemental analysis, molar conductance, powder XRD and SEM. The IR results indicated the tetradentate binding mode of the ligand involving azomethine nitrogen and carboxylato oxygen. The electronic spectral results indicate that Cu(II) complexe has Tetragonally distorted octahedral geometry, while Co(II) and Ni(II) complexes are octahedral. Powder XRD and SEM indicate the nano crystalline state of the complexes. The antimicrobial activity of the synthesized ligand and its complexes were screened by disc diffusion method. The results show that the metal complexes were found to be more active than the ligand. The nuclease activity of the ligand and its complexes were assayed on lamda- DNA using gel electrophoresis in the presence of H2O2. The DNA cleavage activity of complexes is greater than the ligand. The in-vitro anticancer studies reveal that the Co(II) complex had the moderate anticancer activity against HT-29 (Colon Carcinoma) cell with IC50 value of 86.76 mM and Cu(II) complex had IC50 value of 83.24 mM.