The heterocyclic ligand 2-anilino-N'-[(1E)-1-pyridin-2-ylethylidene]acetohydrazide (Apeah) was synthesized by coupling of 2-anilinoacetohydrazide with 2-acetylpyridine and characterized by IR, 1H and 13C-NMR spectra, it was then reacted with Lanthanide(III) nitrates to form complexes of the type [Ln(Apeah)2 NO3 H2O]·2NO3 where, Ln = La(III), Pr(III), Nd(III), Sm(III), Eu(III), Gd(III), Tb(III), Dy(III), Y(III). The metal complexes were characterized on the basis of elemental analysis, molar conductance, magnetic susceptibility measurements, TG/DTA and spectral (IR, 1H and 13C-NMR, UV-Visible and EPR) studies. The spectral data revealed that the Apeah behaves in tridentate fashion coordinating through carbonyl oxygen, azomethine nitrogen and pyridine nitrogen. The molar conductance values adequately supported their 1:2 electrolytic natures. Both Apeah and complexes were screened for their antimicrobial study. An enhancement of antimicrobial activity of the Apeah was observed on complexation.