Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry and Computational Chemistry

Abstract

Synthesis of Salicylic Acid Fused Dihydropyrazole Analogues and their Mechanism of Action on Escherichia coli Cells

Author(s): Naveen Puttaswamy, Rekha ND, Lakshmi Ranganatha V, Bushra Begum , Shaukath Ara Khanum

A series of substituted pyrazoline (9a-j) analogues were synthesized via 1,3-dipolar cycloaddition of substituted 2-benzylidene-1-phenylhydrazine (8a-d) with 5-(3-phenylacryloyl)2-hydroxybenzoic acid (5a-f) and the synthesized compounds were characterized by Infrared (IR), Proton Nuclear Magnetic Resonance (1H-NMR), Carbon-13 Nuclear Magnetic Resonance (13C-NMR) and mass spectral techniques. The antimicrobial activity of all the synthesized compounds were screened for both Gram-positive and Gram-negative bacteria along with a fungal strain and the Minimum Inhibitory Concentration (MIC) was calculated. The toxicity of the compounds on human cells were determined by haemolytic activity. The mechanism of action of antimicrobial activity of the synthesized compounds was evaluated by membrane permeabilization assay and confirmed by scanning electron microscopy. Among the synthesized compounds, compounds 9b, 9f and 9g showed strong plasma membrane-permeabilizing activity, further the membrane disruption was confirmed by large dents as observed in E. coli cells under scanning electron microscopy.


FIND ARTICLES
SCImago Journal & Country Rank
kız uzun zamandır masaj salonunda çalışıyor çok sulanan erkek oluyor ama porno izle bir türlü hiç birine vermiyor Ama sonunda genç paraya sıkışıyor ve bir porno resimleri sikiş için çok para teklif eden genç adama sonunda kabul ediyor ve evlerine giren amatör pornolar maskeli hırsız genç kadının odasındaki çekmecelerde para ararken kadının seksi iç sikiş çamaşırlarına denk geliyor Onları koklayarak yarağı taş gibi oluyor daha sonra genç porno fotoğrafları kadın odaya bir den bire girince hırsızı görüyor ve bağırmaya başlıyor Hırsız hızlıca porno hikayeleri kızın ağzını elleri ile kapatarak tam kızın kalçalarını kucağına alarak sevişiyor
ankara escort
mobile bitcoin casino
Casumo no deposit bonus bitcoin nedir?