Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry and Computational Chemistry

Abstract

The analysis of the plasma vitamin D of the pre-school children

Author(s): Mohamed Kadari, M. Kaid*, B. Dahmani* and K. Allali

The analysis of the plasma vitamin D of the pre-school children in paediatric department, in The University Hospital (CHU) of Tlémcen (Algerian willaya is located in the North West), is carried out by reversed phase high performance liquid chromatography with UV detection. This method is characterized by direct injection of the supernatant obtained by treatment of 100 μL of plasma with the mixture solvent C2H5OH/CH3COOC2H5 (1: 1). The quantification of the concentrations is carried out by using Cholecalciferol as an external standard; elution is made ingredient mode by using mixture CH3OH/CH3CN (70: 30%) and 1.5 mL/mn flow rate. The percentage of deficiency found in this study is 2.63 %.


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