Cardiovascular diseases are the most significant factors leading to death. This study was done to determine the effectShort-term aerobic training on markers of liver, serum lipid and lipoprotein levels in sedentary men. This quasi-experimental study was carried out on 20 non athletic men. Subjects were divided into2 groups: 15 minutes of sit-ups +30 minutes walking group and 30 minutes of sit-ups +15 minutes walking group. High-density lipoprotein (HDL) its sub fractionsHDL2 and HDL3 cholesterol, Total cholesterol (TC),Triglyceride(TG), Aspartate aminotransferase (AST) and Alanine amino transferees(ALT)were evaluated prior and after training for 16 sessions follow: three session in each weeks with one hour aerobic training in each session. Data analyzed using SPSS-18 and independent and dependent T student tests. The differences were not significant of TC, ALT, AST and HDL2 cholesterol observed in 15 minutes of sit-ups +30 minutes walking group and 30 minutes of sit-ups +15 minutes group after training in comparison with prier phase. Significant increase of TG were observed in 30 minutes of situps +15 minutes walking group comparison with prior phase (P<0.01). Significant increase of HDL cholesterol were observed in15 minutes of sit-ups +30 minutes walking group comparison with prier phase (P<0.003). Significant increase of HDL3 cholesterol were observed in 15 minutes of sit-ups +30 minutes walking group and significant reduction 30 minutes of sit-ups +15 minutes walking group after training in comparison with priorphase (P<0.001 and P<0.01).This study showed thatShort-term aerobic training on levels of markers of liver without affecting but positive effects have on the lipid profile changes in non athletic men.