Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry and Computational Chemistry

Abstract

Validated Chiral High Performance Liquid Chromatographic Method for Enantiomeric Separation of Epichlorohydrin on Immobilized mylose Based Stationary Phase

Author(s): Vipul Rane, Vinod Ahirrao, Ravindra Yeole

Chiral Liquid Chromatographic (CLC) method has been established and authenticated for the enantiomeric resolution of epichlorohydrin. Chiral resolution was achieved on Chiralpak-IA immobilized amylose based stationary phase using an eluent consisting of n-hexane:2-propanol (100:2 v/v) and flowing through column at rate of 1.0 ml/min. The column effluent was examined by using UV detector at 205 nm. Resolution between S-(+)-Epichlorohydrin (SE) and R-(-)-Epichlorohydrin (RE) was found to be ≥ 5. The method was authenticated for the quantification of RE in SE. The detector response for RE was proportionate over the concentrations range of 3-40 μg/ml. The Limit of Detection (LOD) and Limit of Quantification (LOQ) for RE were 0.95 μg/ml and 3.2 μg/ml respectively. Average recovery of the RE was in the range of 96.20% to 101.99%. Analytical solutions of both enantiomers in diluent were found to be stable for a day. The proposed method was found to be precise, accurate and robust for quantitative analysis of RE in SE.


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