Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry and Computational Chemistry

Abstract

Validated Chromatographic Method for the Estimation of Ceftazidime and Tazobactam in Pure and Tablet Dosage Form

Author(s): Mohan Gandhi Bonthu, Lakshmana Rao Atmakuri, Venkateswara Rao Jangam

A simple and rapid chromatographic Reverse Phase High Performance Liquid Chromatography (RP-HPLC) method was developed, optimized and validated for the estimation of Ceftazidime (CEF) and Tazobactam (TAZ) simultaneously in pure and tablet dosage form. The selected drugs were analyzed with the aid of Inertsil C18 (250 × 4.6 mm, 5 μ) column using the combination of 0.1 M disodium hydrogen phosphate buffer: acetonitrile: methanol in the ratio of 40:20:40 v/v/v as mobile phase. The wavelength selected for identification is 229 nm. The range for linearity study was fixed as 120-280 μg/ml for CEF and 15-35 μg/ml for TAZ and the elution times were 2.312 and 4.621 min respectively. The r2 values for the selected ranges were found to be greater than 0.99. Repeatability studies showed Relative Standard Deviation (%RSD) less than 2 for both the drugs under all selected concentrations. The accuracy values are 98.11-100.30% for CEF and TAZ respectively. Assay values for the marketed formulation were found to be in the range of 98-102%. The Limit of Detection (LOD) and Limit of Quantification (LOQ) values are2.44 μg/ml and 7.39 μg/ml for CEF and 0.66 μg/ml and 2.02 μg/ml for TAZ respectively. The developed method aptly suits for regular analysis of selected drugs.


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