Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry and Computational Chemistry

Abstract

Zoonotic concern of Campylobacter jejuni in raw and ready-to-eat barbeque chickens along with Egyptian handlers and consumers via molecular and immunofluorescent characterization

Author(s): El Fadaly Hassan Ali, Barakat Ashraf Mohamed Abdel Khalek, Sylvia Osama Ahmed, Abd El-Razik Khaled Atwa, Shimaa Tewfik Omara, Elham Ezzat and Mona S. Zaki

Raw and under cooked barbeque chickenswhich harboring pathogens of public health and zoonotic impact concerning consumers and handler employees. Campylobacter jejuni considers one of the most prevalent chickenborne gastroenteric bacteria. This study spots light on this concept to indemnify zoonotic hazard of C. jejuni by molecular characterization and indirect fluorescent of Egyptian isolates from both chickens and human in contact. From various Egyptian governorates and clinics a total of 588 chicken visceral contents, eviscerated raw and barbeque chickens were collected from different restaurants. Plus, 96 samples from both symptomatic consumers with history of chickens poisoning and chicken handler. Samples were subjected to standard phenotypic identification of C.jejuni, and subsequently immunofluorescent technique (IFT) identification and genetic amplification by PCR using specific primers of hipO gene. The positive results were detected by IFT expressed by green fluorescence staining. PCR amplification of hipO gene. The overall positive ratio of C. jejuni in chicken was 59.2%, where the higher and the lower values were recorded with intestinal contents and barbeque tissues (72.1 and 32.1) respectively. The total positive ratio in contact personals was 51%.Wherever, the higher and the lower values were 75.9% and 40.3% corresponding to symptomatic consumers and handlers employees. Molecular characterization of chicken’s isolates have shown identical fingerprints with human isolates at 323bp, signifying the high possibilities of zoonotic hazards of the collected samples. The present studycan be concluded that the high incidence of C. jejuni in raw and barbeque chickens incriminated in high infection rate within consumers and handlers’ employees’ .This provides background for the design of firm efficient control strategies.


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