Simple Extractive Spectrophotometeric Determination of Amphetamine Sulphate by Acid Dye Complexation in Solid Dosage Form
By Kalkotwar R. S, Kasture A. V., Pattan S. R., Dengale S. S., Dighe N.S.
ABSTRACT
Simple, accurate, rapid and highly sensitive Extractive Spectrophotometeric determination method has been developed and validated for estimation of Amphetamine Sulphate in pharmaceutical solid dosage form. The proposed method is based on the principle that Mono and diamines in the dissociated form (Ion) combined with dissociated form of the Acid dye Solochrome black T. The formation of Acid dye complex has been evaluated by carrying TLC, Infra-red spectra. The acid dye-drug complex has lmax at 520 nm. Beer’s Lambert Law obeyed in the range of 20-100µg ml-1. The results were validated statistically. The further scope for this method is that it can be applied to body fluid in cases of intoxication
Keywords : Spectrophotometric method, Acid-dye complex, Amphetamine Sulphate, Solochrome black T
